Rapid molecular diagnostic test for Zika virus with low demands on sample preparation and instrumentation
The RT-SIBA method is cost-effective and compatible with portable instrumentation, enabling near patient testing and field use.
Diagnosis of Zika virus has so far relied on serological methods which can be time consuming or nucleic acid amplification tests (NAAT) which require high precision instruments and skilled personnel. This paper aimed to investigate a low cost portable NAAT, known as RT-SIBA. Yellow fever, dengue, West Nile, Chikungunya virus, or any of the 15 different unrelated microorganisms were not detected by the RT-SIBA. Three Zika virus specimens that differed in their viral load (high, medium, and low) and a negative specimen were tested. The RT-SIBA Zika virus assay was observed to detect all three Zika virus specimens, with no cross- reaction with the negative specimen. No diagnostic accuracy results were reported.