Single-reaction multiplex reverse transcription PCR for detection of Zika, Chikungunya, and Dengue Viruses

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To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for detecting these viruses.

The detection of Zika Virus using a multiple RT-PCR test (ZCD assay) for Zika, Chikungunya, and Dengue was compared between an existing real time RT-PCR test. The ZCD assay detected 25 of the 26 samples positive by rRT-PCR, whilst confirming 76/107 negative results. Improved sensitivity for Zika virus is needed given the low viremia detected in clinical samples and the current lack of accurate alternative diagnostics. Although preliminary, these data provide evidence for the utility of a multiplex diagnostic test for these pathogens.

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