Rapid molecular detection of Zika Virus in acute-phase urine samples using the recombinase polymerase amplification assay
The development of a recombinase polymerase amplification assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).
A rapid point-of-care test is needed to detect zika virus, especially at low resource settings. A recombinase polymerase amplification assay was developed for zika virus DNA in urine samples. The clinical performance of the assay was evaluated on 25 acute-phase (2-10 days of onset of symptoms) urine samples collected from suspected cases at the Municipal Hospital of Tuparetama, Pernambuco, Brazil. All results were compared with a real-time RT-PCR as gold standard. The limit of the detection of the assay was 21 RNA copies/reaction. The RT-RPA identified 23/25 (Sensitivity: 92%) positive and 9/9 (specificity: 100%) negative samples.
Adolescents, Adults, Both sexes (for groups of both male and female persons), Children, Combatant, Displaced population, Epidemic, Female, Health, Healthcare workers, Host population, Infections and infectious diseases, Internally displaced population, LGBT (lesbian gay bisexual and transgender), Male, Minorities, Neonates/infants, Nervous system and neurological diseases, Non-combatant, Older people, Persons with disabilities, Pregnant women, Prisoners, Returning population, stateless, Viral fevers, Zoonotic and other parasitic infections